Research Faculty

Douglas Borchman, Ph.D.

Professor, Department of Ophthalmology and
Visual Sciences

CONTACT INFORMATION:

301 E. Muhammad Ali Boulevard, Louisville KY 40202
Telephone: 502-852-7435 Fax: 502-852-7450
E-mail: Borchman@louisville.edu

EDUCATION:

RESEARCH INTERESTS:

As a biophysicist, I am equally fascinated by how the human lens can remain clear for decades, as by the factors which cause that clarity to diminish over time. This fascination has led me to explore how the composition, structure and function of membrane lipids contribute to the light scattering with age and cataracts.

The techniques and experience gained from my studies of lens lipids enabled me to study how derangement of tear oils cause dry eye symptoms. In collaboration with Dr. Foulks and Yappert I am using my spectroscopic background in biophysics to follow compositional and conformational changes in tear lipids with age and meibomian gland disease and to diagnose and elucidate the causes of dry eye symptoms.

PUBLICATIONS:

(15 selected publications from 94)

1. Borchman D, Yappert MC: Lipids and the Ocular Lens. J Lipid Res. 2010 Apr 20. [Epub ahead of print] PMID: 20407021.
2. Foulks GN, Borchman D, Yappert M, Sung-Hye K, McKay JW: Topical Azithromycin Therapy of Meibomian Gland Dysfunction: Clinical response and lipid alterations. Cornea, In Press 2010.
3. Borchman D, Foulks GN, Yappert MC, Kakar S, Podoll N, Rychwalski P, Schwietz E: Physical Changes in Human Meibum with Age as Measured by Infrared Spectroscopy. Ophthalmic Res. 2010 Feb 17;44(1):34-42. [Epub ahead of print] PMID: 20160464.
4. Huang L, Yappert MC, Miller JJ, Borchman D: Thyroxine Ameliorates Oxidative Stress by Inducing Lipid Compositional Changes in Human Lens Epithelial Cells. Invest Ophthalmol Vis Sci. 2007 Aug;48(8):3698-704. PMID: 17652741.
5. Marian MJ, Liu L, Mukhopadhyay P, Paterson CA, Borchman D: Regulation of Sarco/Endoplasmic and Plasma Membrane Calcium ATPase Gene Expression by Calcium in Cultured Human Lens Epithelial Cells. Cell Calcium. 2007 Jan;41(1):87-95. Epub 2006 Jul 26. PMID: 16875731.
6. Huang L, Tang D, Yappert MC, Borchman D: Oxidation Induced Changes in Human Lens Epithelial Cells. 2. Mitochondria and the Generation of Reactive Oxygen Species. Free Radic Biol Med. 2006 Sep 15;41(6):926-36. Epub 2006 May 26. PMID: 16934675.
7. Estrada R, Borchman D, Reddan J, Hitt A, Yappert MC: In-Vitro and in-Situ Tracking of Choline-Phospholipid Biogenesis by MALDI TOF-MS. Anal Chem. 2006 Feb 15;78(4):1174-80. PMID: 16478109
8. Tang D, Dean WL, Borchman D, and Paterson CA: The Influence of Membrane Lipid Structure on Plasma Membrane Ca2+-ATPase Activity. Cell Calcium. 2006 Mar;39(3):209-16. Epub 2006 Jan 10. PMID: 16412504
9. Huang L, Grami V, Marrero Y, Tang D, Yappert MC, Rasi V, Borchman D: Human Lens Phospholipid Changes with Age and Cataract. Invest Ophthalmol Vis Sci. 2005 May;46(5):1682-9. PMID: 15851569
10. Borchman D, Yappert MC, Afzal M.: Lens lipids and maximum lifespan. Exp Eye Res. 2004 Dec;79(6):761-8. PMID: 15642313
11. Zeng J, Zhang Z, Paterson CA, Ferguson-.Yankey S, Yappert MC, Borchman D: Ca2+ -ATPase activity and lens lipid composition in reconstituted systems. Exp Eye Res. 1999 Sep;69(3):323-30. PMID: 10471340
12. Borchman D, Tang D, Yappert MC: Lipid composition, membrane structure relationships in lens and muscle sarcoplasmic reticulum. Biospectroscopy. 1999;5(3):151-67. PMID: 10380082
13. Tang D, Borchman D: Temperature induced structural changes of a crystallin and sphigomyelin binding. Exp Eye Res. 1998 Jul;67(1):113-8. PMID: 9702184
14. Paterson CA, Zeng J, Hussenini Z, Borchman D, Delamere NA, Garland D, Jimenez-Asenio J.: Calcium ATPase activity and membrane structure in clear and cataractous human lenses. Curr Eye Res. 1997 Apr;16(4):333-8. PMID: 9134322
15. Ferguson SR, Borchman D, Yappert MC: Confirmation of the identity of the major phospholipid in human lens membranes. Invest Ophthalmol Vis Sci. 1996 Jul;37(8):1703-6. PMID: 8675415